The improved clarity of the supernatant broth permits earlier detection of growth especially when combined with the increased growth of most organisms. The improved growth also enhances GLC identification of anaerobic bacteria. The addition of glucose to the formulation allows rapid, heavy growth of anaerobic bacteria in a short time and leads to a more rapid identification of important anaerobes. The products of growth do not rapidly destroy the inoculated organisms and therefore it is an excellent medium for the storage of aerobic and anaerobic bacteria. Mixed cultures of bacteria survive in Cooked Meat Medium without displacing the slower growing organisms. It has the ability to initiate bacterial growth from very small inocula and to maintain the viability of cultures over long periods of time. Do not cool the bottles rapidly because ebullition will expel the meat particles from the containers.Ĭooked Meat Medium prepared from heart tissue is a well established medium for the cultivation of anaerobic and aerobic organisms 1. Sterilise by autoclaving at 121☌ for 15 minutes. Allow to stand for 15 minutes until the meat particles are thoroughly wetted. Suspend 10g in 100ml of distilled water (or 1g amounts in 10ml volumes of water in tubes). * Adjusted as required to meet performance standards An excellent medium for the primary growth and maintenance of aerobic and anaerobic organisms